oncotomskСибирский онкологический журналSiberian journal of oncology1814-48612312-3168Tomsk National Research Medical Сепtеr of the Russian Academy of Sciencesoncotomsk-141Research ArticleЛАБОРАТОРНЫЕ И ЭКСПЕРИМЕНТАЛЬНЫЕ ИССЛЕДОВАНИЯLABORATORY AND EXPERIMENTAL STUDIESВЛИЯНИЕ РЫБЬЕГО ЖИРА НА РАЗВИТИЕ ОПУХОЛЕЙ МОЛОЧНОЙ ЖЕЛЕЗЫ И ТОЛСТОЙ КИШКИ, ВЫЗЫВАЕМЫХ МЕТИЛНИТРОЗОМОЧЕВИНОЙ В ОРГАНАХ-МИШЕНЯХ У КРЫСEFFECT OF FISH OIL ON DEVELOPMENT OF MAMMARY GLAND AND COLON TUMORS INDUCED BY METHYLNITROSOUREA IN TARGET ORGANS OF RATSБеспаловВ. Г.BespalovV. G.

Беспалов Владимир Григорьевич - доктор медицинских наук, руководитель лаборатории химиопрофилактики рака и онкофармакологии.E-mail: bespalov_niio@mail.ru. SPIN-код РИНЦ: 1920-1515

Bespalov Vladimir Grigorevich - MD, DSc, Professor. E-mail: bespalov_niio@mail.ru

bespalov_niio@mail.ruАлександровВ. А.AlexandrovV. A.

Александров Валерий Анатольевич - доктор медицинских наук, профессор, ведущий научный сотрудник лаборатории химиопрофилактики рака и онкофармакологии.E-mail: alexandrov.valeri@yandex.ru

Aleksandrov Valerij Anatolevich - MD, DSc, Professor, Principal Investigator.E-mail: alexandrov.valeri@yandex.ru

alexandrov.valeri@yandex.ruНаучно-исследовательский институт онкологии имени Н.Н. Петрова, Санкт-ПетербургРоссияN.N. Petrov Research Institute of Oncology of the Ministry of Health, St. PetersburgRussian Federation201518022016125357Copyright © Беспалов В.Г., Александров В.А., 20162016Беспалов В.Г., Александров В.А.Bespalov V.G., Alexandrov V.A.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.siboncoj.ru/jour/article/view/141

Изучен игибирующий эффект рыбьего жира на развитие опухолей молочной железы и толстой кишки на модели многоорганного канцерогенеза, индуцируемого метилнитрозомочевиной (МНМ) у взрослых крыс-самок. МНМ растворяли в физиологическом растворе и вводили непосредственно в органымишени: однократные инъекции по 1 мг в каждую из 12 молочных желез, и этим же крысам делали 4 интраректальные инстилляции по 4 мг 1 раз в нед. В последующие 6 мес крысам вводили перорально зондом рыбий жир или подсолнечное масло (для сравнения): 5 дней в нед по 1 мл в день. К концу экспериментов (7 мес) в результате воздействия МНМ у крыс обнаружены опухоли в 85,7–92,9 %, причем более половины из них – множественные опухоли (чаще аденокарциномы) молочной железы и толстой кишки. Подсолнечное масло не оказывало значимого эффекта на канцерогенез. Рыбий жир по сравнению с группой МНМ-контроль значимо уменьшал частоту опухолей молочной железы – в 1,6 раза и их множественность – в 3,1 раза. Влияние рыбьего жира на развитие опухолей толстой кишки выразилось в уменьшении множественности новообразований в 1,6 раза, причем только по сравнению с группой МНМ + масло. Представляется перспективным дальнейшее изучение и применение рыбьего жира в качестве онкопротектора.

In experiments with adult female rats was studied effect of fish oil on development of mammary gland and colon tumors induced by methylnitrosourea (MNU) using a multi-organ carcinogenesis model. MNU in saline solution administered directly into the target organs: one injection of 1 mg locally in each 12 mammary glands and four intrarectal instillations of 4 mg one time per week. Then rats were given fish oil or sunflower oil (control), 1 ml/rat per day five times a week by gavage for the consecutive 6 months. To the end of experiments when rats were sacrificed in groups with treatment of MNU tumors were detected in 85,7–92,9 %, more half of its were multiple tumors (more often adenocarcinomas) of mammary gland and colon. Sunflower oil did not have significant effect on the carcinogenesis. In rats from fish oil + MNU group compared with the MNU-control group the incidence and multiplicity of mammary tumors were significantly decreased by 1.6 and 3.1 times, correspondingly. Fish oil had less inhibitory effect on the development of colon tumors: decrease of tumor multiplicity (by 1.6 times) was detected only in comparison with MNU + sunflower oil group. These results are interesting for consequence study and use of fish oil as oncoprotector.

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The authors declare that there are no conflicts of interest present.