oncotomskСибирский онкологический журналSiberian journal of oncology1814-48612312-3168Tomsk National Research Medical Сепtеr of the Russian Academy of Sciencesoncotomsk-148Research ArticleКЛИНИЧЕСКИЕ ИССЛЕДОВАНИЯCLINICAL STUDIESОЦЕНКА ЧУВСТВИТЕЛЬНОСТИ К АНТИБИОТИКАМ АНАЭРОБНЫХ ВОЗБУДИТЕЛЕЙ BACTEROIDES, PREVOTELLA И FUSOBACTERIUM, ВЫДЕЛЕННЫХ ОТ ОНКОЛОГИЧЕСКИХ БОЛЬНЫХEVALUATION OF ANTIBIOTIC SUSCEPTIBILITY OF ANAEROBIC PATHOGENS BACTEROIDES, PREVOTELLA AND FUSOBACTERIUM ISOLATED FROM CANCER PATIENTSШильниковаИ. И.ShilnikovaI. I.

Шильникова Ирина Ивановна, кандидат биологических наук, старший научный сотрудник лаборатории микробиологической диагностики и лечения инфекций в онкологии.

Тел.: +7 (967) 084-76-70. E-mail: iish54@mail.ru. SPIN-код: 9400-6222 

Shilnikova Irina Ivanovna, PhD, Senior Researcher of Laboratory for Microbiologic Diagnosis and Treatment of Infection in Oncology.

Phone: +7 (967) 084-76-70. E-mail: iish54@ mail.ru. SPIN-code: 9400-6222

iish54@mail.ruДмитриеваН. В.DmitrievaN. V.

Дмитриева Наталья Владимировна, доктор медицинских наук, профессор, заведующая лабораторией микробиологической диагностики и лечения инфекций в онкологии.

Тел.: +7 (499) 324-18-40. E-mail: prof.ndmitrieva@mail.ru

Dmitrieva Natalia Vladimirovna, MD, DSc, Professor, Head of Laboratory for Microbiologic Diagnosis and Treatment of Infection in Oncology.

Phone: +7 (499) 324-18-40. E-mail: prof. ndmitrieva@mail.ru

prof.ndmitrieva@mail.ruРоссийский онкологический научный центр им. Н.Н. Блохина, МоскваРоссияN.N. Blokhin Russian Cancer Research Center of the Russian Academy of Medical Sciences, MoscowRussian Federation201518022016153743Copyright © Шильникова И.И., Дмитриева Н.В., 20162016Шильникова И.И., Дмитриева Н.В.Shilnikova I.I., Dmitrieva N.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.siboncoj.ru/jour/article/view/148

В исследовании было проанализировано 122 штамма строго анаэробных грамотрицательных палочек рода Bacteroides, Prevotella и Fusobacterium, выделенных от онкологических больных в 2004–2014 гг. Большинство штаммов принадлежало к группе Bacteroides fragilis (55 %), затем по частоте выделения следовали штаммы Prevotella (34,4 %) и Fusobacterium (10,6 %). Видовую идентификацию штаммов проводили с помощью времяпролетной матрично-ассоциированной лазерной десорбционноионизационной масс-спектрометрии (MALDI-TOF MS). Из группы B. fragilis наиболее часто выделялись B. fragilis, B. thetaiotaomicron, B. ovatus и В. vulgatus. Среди Prevotella наиболее часто выделялись Р. buccae, Р. buccalis, Р. оris, Р. denticola и P. nigrescens, а среди Fusobacterium 77 % составляли штаммы F. nucleatum. Чувствительность выделенных штаммов к антимикробным препаратам определяли методом E-теста. Процент чувствительности изолятов группы B. fragilis составлял для метронидазола 97 %; имипенема – 95,5 %; амоксициллин / клавуланата – 95,5 % и клиндамицина – 77,6 %. Три штамма B. fragilis имели множественную резистентность (одновременно к имипенему, амоксициллин / клавуланату и метронидазолу либо клиндамицину). Все выделенные штаммы Prevotella были чувствительны к имипенему и амоксициллин / клавуланату, в то время как 78,6 % пигментных видов Prevotella и 46,4 % непигментированных видов были устойчивы к пенициллину. Количество штаммов, чувствительных к метронидазолу и клиндамицину, составляло 93 и 88 % соответственно. Все штаммы Fusobacterium были чувствительны ко всем тестируемым антибиотикам, включая пенициллин.

In this study 122 strains of strictly anaerobic gram-negative rods Bacteroides, Prevotella and Fusobacterium, isolated from cancer patients between 2004 and 2014 were analyzed. The most common strains were B. fragilis (55 %) followed by Prevotella (34.4 %) and Fusobacterium spp (10.6 %). The species identification was carried out by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS). Among the B. fragilis group strains, B. fragilis, B. thetaiotaomicron, B. ovatus and B. vulgatus were isolated most frequently. Among Prevotella species, the most frequently isolated species were P. buccae, P. buccalis, P. oris, P. denticola and P. nigrescens, and 77 % of the Fusobacterium spp. were F. nucleatum. Susceptibilities of the isolates to antimicrobial agents were determined by the E-test methodology. The percentage of the susceptibility of B. fragilis group isolates were: metronidazole, 97 %; imipenem, 95.5 %; amoxicillin/clavulanate, 95.5 % and clindamycin, 77.6 %. Three B. fragilis isolates proved to be multidrug-resistant (simultaneously to imipenem, amoxicillin/ clavulanate and metronidazole or clindamycin). All Prevotella isolates were susceptible to imipenem and amoxicillin/clavulanate, whereas 78.6 % of the pigmented Prevotella species and 46.4 % of the non-pigmented species were resistant to penicillin. The number of strains susceptibility to metronidazole and clindamycin were 93 % and 88 %, respectively. All Fusobacterium strains were sensitive to all tested antibiotics, including penicillin.

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The authors declare that there are no conflicts of interest present.