oncotomskСибирский онкологический журналSiberian journal of oncology1814-48612312-3168Tomsk National Research Medical Сепtеr of the Russian Academy of Sciences10.21294/1814-4861-2022-21-6-91-98oncotomsk-2378Research ArticleЛАБОРАТОРНЫЕ И ЭКСПЕРИМЕНТАЛЬНЫЕ ИССЛЕДОВАНИЯLABORATORY AND EXPERIMENTAL STUDIESКуркумин снижает пролиферацию клеток рака молочной железы через белки RASSF1A, Bax и caspase-3Curcumin as an anti-proliferative agent in breast cancer through RASSF1A, Bax, and caspase-3 proteinhttps://orcid.org/0000-0002-7213-3270RahmahN. A.RahmahN. A.

Nunung Ainur Rahmah, dr., Sp.PA, кафедра анатомической патологии, медицинский факультет, 

Джакарта, 10520

Nunung Ainur Rahmah, dr., Sp.PA, Department of anatomical pathology, Faculty of Medicine, 

Jakarta, 10520

nunung.rahmah@klinikjurnal.comhttps://orcid.org/0000-0003-1614-4108HarliansyahH.HarliansyahH.

Harliansyah, M.Si., PhD, кафедра биохимии, медицинский факультет, 

Джакарта, 10520

Harliansyah, M.Si., PhD, Department of biochemistry, Faculty of Medicine,

Jakarta, 10520

https://orcid.org/0000-0002-7168-5743SuyatnaF. D.SuyatnaF. D.

Franciscus D. Suyatna, Prof., dr., PhD., Sp.FK, кафедра фармакологии, медицинский факультет, 

Джакарта, 10440

Franciscus D. Suyatna, Prof., dr., PhD., Sp.FK, Department of pharmacology, Faculty of Medicine, 

Jakarta, 10440

https://orcid.org/0000-0002-2891-3077KanokoM.KanokoM.

Mpu Kanoko, Prof., dr., SpPA(K), PhD, кафедра анатомической патологии, медицинский факультет, 

Джакарта, 10440

Mpu Kanoko, Prof., dr., SpPA(K), PhD, Department of anatomical pathology, Faculty of Medicine,

Jakarta, 10440

https://orcid.org/0000-0003-2325-1091RustamadjiP.RustamadjiP.

Primariadewi Rustamadji, Dr. dr., MM. SpPA(K), кафедра анатомической патологии, медицинский факультет, 

Джакарта, 10440

Primariadewi Rustamadji, Dr. dr., MM. SpPA(K), Department of anatomical pathology, Faculty of Medicine,

Jakarta, 10440

https://orcid.org/0000-0001-5684-9693PrihartonoJ.PrihartonoJ.

Joedo Prihartono, Dr. dr., MPH, кафедра здравоохранения, медицинский факультет, 

Джакарта, 10440

Joedo Prihartono, Dr. dr., MPH, Department of Public health, Faculty of Medicine, 

Jakarta, 10440

https://orcid.org/0000-0001-9222-6610BustamiA.BustamiA.

Arleni Bustami, MS. Ka, кафедра объединенной лаборатории, медицинский факультет, 

Джакарта, 10440

Arleni Bustami, MS. Ka, Department of Integrated laboratory, Faculty of Medicine,

Jakarta, 10440

https://orcid.org/0000-0002-6655-0633HaryonoS. J.HaryonoS. J.

Samuel Johny Haryono, Dr. dr., Sp.B (K)Onk, отделение онкологии, 

Джакарта, 11420

Samuel Johny Haryono, Dr. dr., Sp.B (K)Onk, Department of oncology, 

Jakarta, 11420

https://orcid.org/0000-0003-2426-2534HernowoB. S.HernowoB. S.

Hj. Bethy S. Hernowo, Prof., dr., Sp.PA(K), PhD, кафедра анатомической патологии, медицинский факультет, 

Бандунг, 45363

Hj. Bethy S. Hernowo, Prof., dr., Sp.PA(K), PhD, Department of anatomical pathology, Faculty of Medicine,

Bandung, 45363

Университет ЯрсиИндонезияUniversitas YARSIIndonesiaУниверситет ИндонезииИндонезияUniversitas IndonesiaIndonesiaБольница Национального онкологического центра DharmaisИндонезияNational Cancer Center Dharmais HospitalIndonesiaУниверситет ПаджаджаранИндонезияUniversitas PadjajaranIndonesia2022311220222169198Copyright © Rahmah N.A., Harliansyah H., Suyatna F.D., Kanoko M., Rustamadji P., Prihartono J., Bustami A., Haryono S.J., Hernowo B.S., 20232023Rahmah N.A., Harliansyah H., Suyatna F.D., Kanoko M., Rustamadji P., Prihartono J., Bustami A., Haryono S.J., Hernowo B.S.Rahmah N.A., Harliansyah H., Suyatna F.D., Kanoko M., Rustamadji P., Prihartono J., Bustami A., Haryono S.J., Hernowo B.S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.siboncoj.ru/jour/article/view/2378Актуальность

Актуальность. Куркумин представляет собой полифенол, обладающий фармакологической активностью, которая может ингибировать рост опухолевых клеток и вызывать апоптоз с помощью различных механизмов. Однако конкретный механизм цитотоксичности куркумина остается спорным из-за множества анти- и проапоптотических сигнальных путей в различных типах клеток.

Целью исследования явилось изучение взаимосвязи куркумина с уровнями белка RASSF1A, Bax и активностью каспазы-3 в поддержке механизма апоптоза в клетках рака молочной железы CSA03.

Материал и методы

Материал и методы. Введение куркумина в раковые клетки основано на различиях в дозировке при 24-часовой инкубации. Цитотоксичность после введения куркумина определяли с помощью MTS. Уровни белков RASSF1A и Bax тестировали с помощью ELISA. Активность каспазы-3 использовали для определения апоптоза и тестировали с помощью проточной цитометрии. Статистическая обработка данных проводилась с использованием программы Statistical Program for Social Science (SPSS) версии 20.0 для Windows. Проверка гипотез выполнялась с использованием непараметрического численного сравнительного теста проверки гипотез. Для оценки разницы между >2 группами непарных концентраций использовали непараметрический критерий Крускала–Уоллиса, а для оценки значимой разницы в двух группах проводили апостериорный анализ Манна–Уитни.

Результаты

Результаты. Показано, что цитотоксический эффект куркумина составил 40,85 мкг/мл. В концентрациях 40 мкг/мл и 50 мкг/мл куркумин увеличивает уровни белка RASSF1A (∆ = 26,53 % и 47,35 %, соответственно), Bax (∆ = 48,79 % и 386,15 %) и каспазы-3 (∆ = 1678,51 % и 1871,889 %).

Заключение

Заключение. Введение куркумина в клетки CSA03 эффективно уменьшало рост клеток рака молочной железы in vitro за счет реактивации RASSF1A в качестве гена-супрессора опухоли и повышало уровень белка Bax в качестве индукции апоптоза (caspase-3). 

Background

Background. Curcumin is a polyphenol that has pharmacological activity that can inhibit tumor cell growth and induce apoptosis through various mechanisms. However, the specifc mechanism of curcumin cytotoxicity remains controversial because of many anti- and pro-apoptotic signaling pathways in various cell types.

This study aims to examine the relationship among curcumin on RASSF1A, Bax protein levels, and caspase-3 activity in supporting the apoptotic mechanism in CSA03 breast cancer cells.

Material and Methods

Material and Methods. Curcumin administration to cancer cells is based on differences in dosage with 24-hour incubation. Cytotoxicity after curcumin administration was determined using MTS. RASSF1A and Bax protein levels were tested through ELISA. Caspase-3 activity was used to determine apoptosis and was tested using fow cytometry.

Results

Results. The results indicated that curcumin had a cytotoxicity effect of 40.85 µg/mL. At concentrations of 40 µg/mL and 50 µg/mL, curcumin increases levels of protein RASSF1A (∆ = 26.53% and 47.35%, respectively), Bax (∆ = 48.79% and 386.15%), and caspase-3 (∆ = 1,678.51% and 1,871.889%) signifcantly.

Conclusions

Conclusions. Curcumin exhibits anti-proliferative activity and apoptotic (Caspase-3) effects through activation of RASSF1A and Bax. 

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The authors declare that there are no conflicts of interest present.