oncotomskСибирский онкологический журналSiberian journal of oncology1814-48612312-3168Tomsk National Research Medical Сепtеr of the Russian Academy of Sciences10.21294/1814-4861-2024-23-5-73-84oncotomsk-3270Research ArticleЛАБОРАТОРНЫЕ И ЭКСПЕРИМЕНТАЛЬНЫЕ ИССЛЕДОВАНИЯLABORATORY AND EXPERIMENTAL STUDIESБиологическое тестирование никелидтитановых имплантатов с покрытиями из благородных металлов в эксперименте in vivoBiological testing of titanium-containing implants with noble metal coatings in an in vivo experimenthttps://orcid.org/0000-0003-3047-4613ЖеравинА. А.ZheravinA. A.

Жеравин Александр Александрович, кандидат медицинских наук, исполнитель РНФ проекта № 20-15-00222-П во временном научном коллективе № 642, 630090, г. Новосибирск, пр. акад. Лаврентьева, 3;

заведующий научно-исследовательским отделом онкологии и радиотерапии, онколог, 630055, г. Новосибирск, ул. Речкуновская, 15

Alexander A. Zheravin, MD, PhD, RSF executor of project No. 20-15-00222-P in temporary research team No. 642, 3, Lavrentyev Ave., Novosibirsk, 630090;

Head of the Oncology and Radiotherapy Research Department, Oncologist, 15, Rechkunovskaya St., Novosibirsk, 630055

zheravin2010@yandex.ruhttps://orcid.org/0000-0002-1314-3065ДоровскихС. И.DorovskikhS. I.

Доровских Светлана Игоревна, кандидат химических наук, научный сотрудник лаборатории 314, 

630090, г. Новосибирск, пр. акад. Лаврентьева, 3

Svetlana I. Dorovskikh, PhD, Researcher, Laboratory 314,

3, Lavrentyev Ave., Novosibirsk, 630090

https://orcid.org/0000-0001-8574-7522ВикуловаЕ. С.VikulovaE. S.

Викулова Евгения Сергеевна, кандидат химических наук, старший научный сотрудник, заведующая лабораторией 314, 

630090, г. Новосибирск, пр. акад. Лаврентьева, 3

Eugenia S. Vikulova, PhD, Senior Researcher, Head of Laboratory 314,

3, Lavrentyev Ave., Novosibirsk, 630090

https://orcid.org/0000-0003-4001-8371БасоваТ. В.BasovaT. V.

Басова Тамара Валерьевна, доктор химических наук, профессор РАН, главный научный сотрудник, заведующая лабораторией 313, 

630090, г. Новосибирск, пр. акад. Лаврентьева, 3

Tamara V. Basova, DSc, Professor of the Russian Academy of Sciences, Chief Researcher, Head of Laboratory 313,

3, Lavrentyev Ave., Novosibirsk, 630090

https://orcid.org/0000-0001-8393-8210ВасильеваМ. Б.VasilevaM. B.

Васильева Мария Борисовна, кандидат медицинских наук, старший преподаватель кафедры фундаментальной медицины, Институт медицины и психологии В. Зельмана, 

630090, г. Новосибирск, ул. Пирогова, 1

Maria B. Vasileva, MD, PhD, Senior Lecturer, Department of Fundamental Medicine, 

1, Pirogova St., Novosibirsk, 630090

https://orcid.org/0000-0003-1994-5787РусаковаЯ. Л.RusakovaYa. L.

Русакова Янина Леонидовна, кандидат ветеринарных наук, ведущий научный сотрудник экспериментально-биологической клиники Института экспериментальной биологии и медицины, 

630055, г. Новосибирск, ул. Речкуновская, 15

Yanina L. Rusakova, MD, PhD, Leading Researcher, Experimental Biological Clinic, Institute of Experimental Biology and Medicine,

15, Rechkunovskaya St., Novosibirsk, 630055

https://orcid.org/0000-0003-3739-4982МорозоваН. Б.MorozovaN. B.

Морозова Наталья Борисовна, доктор химических наук, главный научный сотрудник лаборатории 313, 

630090, г. Новосибирск, пр. акад. Лаврентьева, 3

Nataliya B. Morozova, DSc, Chief Researcher of Laboratory 313, 

3, Lavrentyev Ave., Novosibirsk, 630090

Институт неорганической химии им. А.В. Николаева СО РАН; ФГБУ «Национальный медицинский исследовательский центр им. ак. Е.Н. Мешалкина» Минздрава РоссииРоссияA.V. Nikolaev Institute of Inorganic Chemistry SB RAS; E.N. Meshalkin National Medical Research Center of the Ministry of Health of RussiaRussian FederationИнститут неорганической химии им. А.В. Николаева СО РАНРоссияA.V. Nikolaev Institute of Inorganic Chemistry SB RASRussian FederationФГАОУ ВО «Новосибирский национальный исследовательский государственный университет»РоссияNovosibirsk National Research State UniversityRussian FederationФГБУ «Национальный медицинский исследовательский центр им. ак. Е.Н. Мешалкина» Минздрава РоссииРоссияE.N. Meshalkin National Medical Research Center of the Ministry of Health of RussiaRussian Federation2024101120242357384Copyright © Жеравин А.А., Доровских С.И., Викулова Е.С., Басова Т.В., Васильева М.Б., Русакова Я.Л., Морозова Н.Б., 20242024Жеравин А.А., Доровских С.И., Викулова Е.С., Басова Т.В., Васильева М.Б., Русакова Я.Л., Морозова Н.Б.Zheravin A.A., Dorovskikh S.I., Vikulova E.S., Basova T.V., Vasileva M.B., Rusakova Y.L., Morozova N.B.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.siboncoj.ru/jour/article/view/3270

Цель исследования – провести биологическое in vivo тестирование образцов никелида титана, модифицированных пленочными гетероструктурами Ag/Pt или AuAg/Pt, в сравнении с исходным носителем.

Материал и методы

Материал и методы. Объекты исследования – пластины из никелида титана, модифицированные пленочными гетероструктурами из благородных металлов, в качестве тест-системы для in vivo тестирования использовали лабораторную мини-свинью (мини-пиг). Для формирования пленочных структур на образцах никелида титана использованы физические методы газофазного осаждения: ионно-плазменное (IPD) и термическое (PVD) напыление. Гетероструктуры Ag/Pt и AuAg/Pt охарактеризованы методами рентгеновской дифракции и сканирующей микроскопии.

Результаты

Результаты. Биосовметимость имплантатов до (TiNi, контроль) и после (Ag/Pt/TiNi и AuAg/Pt/TiNi) модификации была проверена в in vivo эксперименте на лабораторном животном (мини-пиг). Общетоксические реакции организма на введенные образцы отсутствуют. Был проведен сравнительный макроскопический и гистологический анализ состояния периимплантных тканей после 39 сут имплантации. В соединительнотканой капсуле вокруг образца TiNi было выявлено наличие некоторого количества лимфоцитов, эозинофилов и макрофагов, но эти показатели снижаются в ряду TiNi > AuAg/Pt/TiNi > Ag/Pt/TiNi.

Заключение

Заключение. Продемонстрирован положительный эффект модификации поверхности никелида титана гетероструктурами из благородных металлов на биосовместимость металлических имплантатов в эксперименте in vivo.

The aim of study is to conduct in vivo biological testing of titanium nickelide samples modifed with Ag/Pt or AuAg/Pt flm heterostructures in comparison with the bare carrier.

Material and Methods

Material and Methods. Titanium nickelide plates modifed with flm heterostructures made of noble metals and the laboratory mini-pigs used for in vivo tests were the objects of the study. To form flm structures on titanium nickelide samples, the physical gasphase deposition methods: ion plasma deposition (IPD) and thermal (PVD) sputtering were used. The Ag/ Pt or AuAg/Pt heterostructures were characterized by X-ray diffraction and scanning microscopy methods.

Results

Results. The biocompatibility of implants before (TiNi, control) and after (Ag/Pt/TiNi and AuAg/Pt/TiNi) modifcation with flm heterostructures was tested in in-vivo experiments on a laboratory animal (mini-pig). General toxic reactions of the body to the injected samples were absent. A comparative macroscopic and histological analysis of the condition of peri-implant tissues after 39 days of implantation was performed. The connective tissue capsule around the TiNi sample revealed the presence of a certain number of lymphocytes, eosinophils and macrophages, but these indicators decrease in the order of TiNi > AuAg/Pt/TiNi > Ag/Pt/TiNi.

Conclusion

Conclusion. The positive effect of modifying the titanium nickelide surfaces with noble metal heterostructures on the biocompatibility of metal implants was demonstrated in an in vivo experiment.

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The authors declare that there are no conflicts of interest present.