oncotomskСибирский онкологический журналSiberian journal of oncology1814-48612312-3168Tomsk National Research Medical Сепtеr of the Russian Academy of Sciences10.21294/1814-4861-2024-23-6-97-106oncotomsk-3358Research ArticleЛАБОРАТОРНЫЕ И ЭКСПЕРИМЕНТАЛЬНЫЕ ИССЛЕДОВАНИЯLABORATORY AND EXPERIMENTAL STUDIESЭкспрессия C/D мякРНК в клеточных линиях лейкоза при хромосомных аномалиях после облученияsnoRNA box C/D levels in leukemia cells in chromosomal abnormalities after irradiationhttps://orcid.org/0000-0003-1518-4677РасторгуеваЕ. В.RastorguevaE. V.

Расторгуева Евгения Владимировна - младший научный сотрудник, Научно-исследовательский технологический институт им. С.П. Капицы.

432017, Ульяновск, ул. Льва Толстого, 42

Researcher ID (WOS) F-9859-2019, Author ID (Scopus) 36137821200

Evgenia V. Rastorgueva - Junior Researcher, S.P. Kapitsa National Research Institute of Technology, Ulyanovsk State University.

42, Leo Tolstoy St., Ulyanovsk, 432017

Researcher ID (WOS) F-9859-2019, Author ID (Scopus) 36137821200

rastorgueva.e.v@yandex.ruhttps://orcid.org/0000-0001-8183-5103ПогодинаЕ. С.PogodinaE. S.

Погодина Евгения Сергеевна - кандидат биологических наук, научный сотрудник, Научно-исследовательский технологический институт им. С.П. Капицы.

432017, Ульяновск, ул. Льва Толстого, 42

Researcher ID (WOS) E-9244-2014, Author ID (Scopus) 57194655594

Evgenia S. Pogodina - PhD, Researcher, S.P. Kapitsa National Research Institute of Technology, Ulyanovsk State University.

42, Leo Tolstoy St., Ulyanovsk, 432017

Researcher ID (WOS) E-9244-2014, Author ID (Scopus) 57194655594

https://orcid.org/0000-0001-7484-2671ЮроваЕ. В.IurovaE. V.

Юрова Елена Валерьевна - младший научный сотрудник, Научно-исследовательский технологический институт им. С.П. Капицы.

432017, Ульяновск, ул. Льва Толстого, 42

Researcher ID (WOS) D-6956-2017, Author ID (Scopus) 57219328888

Elena V. Iurova - Junior Researcher, S.P. Kapitsa National Research Institute of Technology, Ulyanovsk State University.

42, Leo Tolstoy St., Ulyanovsk, 432017

Researcher ID (WOS) D-6956-2017, Author ID (Scopus) 57219328888

https://orcid.org/0000-0002-5666-5154БелобородовЕ. A.BeloborodovE. A.

Белобородов Евгений Алексеевич - аспирант, научный сотрудник, Научно-исследовательский технологический институт им. С.П. Капицы.

432017, Ульяновск, ул. Льва Толстого, 42

Researcher ID (WOS) E-8072-2017, Author ID (Scopus) 57194654215

Evgeniy A. Beloborodov - Postgraduate, Researcher, S.P. Kapitsa National Research Institute of Technology, Ulyanovsk State University.

42, Leo Tolstoy St., Ulyanovsk, 432017

Researcher ID (WOS) E-8072-2017, Author ID (Scopus) 57194654215

https://orcid.org/0000-0002-3276-8976СугакД. Е.SugakD. E.

Сугак Дмитрий Евгеньевич - младший научный сотрудник, Научно-исследовательский технологический институт им. С.П. Капицы.

432017, Ульяновск, ул. Льва Толстого, 42

Dmitry E. Sugak - Junior Researcher, S.P. Kapitsa National Research Institute of Technology, Ulyanovsk State University.

42, Leo Tolstoy St., Ulyanovsk, 432017

https://orcid.org/0009-0002-6107-9261ТумозовИ. А.TumozovI. A.

Тумозов Иван Андреевич - стажер-исследователь, Научно-исследовательский технологический институт им. С.П. Капицы.

432017, Ульяновск, ул. Льва Толстого, 42

Ivan A. Tumozov - Research Intern, S.P. Kapitsa National Research Institute of Technology, Ulyanovsk State University.

42, Leo Tolstoy St., Ulyanovsk, 432017

https://orcid.org/0000-0002-4402-1482СаенкоЮ. В.SaenkoYu. V.

Саенко Юрий Владимирович - доктор биологических наук, профессор, ведущий научный сотрудник, Научно-исследовательский технологический институт им. С.П. Капицы.

432017, Ульяновск, ул. Льва Толстого, 42

Researcher ID (WOS) D-9789-2014, Author ID (Scopus) 10440760400

Yuri V. Saenko - DSc, Professor, Leading Researcher, S.P. Kapitsa National Research Institute of Technology, Ulyanovsk State University.

42, Leo Tolstoy St., Ulyanovsk, 432017

Researcher ID (WOS) D-9789-2014, Author ID (Scopus) 10440760400

https://orcid.org/0000-0003-0826-1857ФоминА. Н.FominA. Ni.

Фомин Александр Николаевич - кандидат технических наук, директор, старший научный сотрудник, Научно-исследовательский технологический институт им. С.П. Капицы.

432017, Ульяновск, ул. Льва Толстого, 42

Author ID (Scopus) 56973686300

Alexander N. Fomin - PhD, Director, Senior Researcher, S.P. Kapitsa National Research Institute of Technology, Ulyanovsk State University.

42, Leo Tolstoy St., Ulyanovsk, 432017

Author ID (Scopus) 56973686300

ФГБОУ ВО «Ульяновский государственный университет»РоссияUlyanovsk State UniversityRussian Federation20241201202523697106Copyright © Расторгуева Е.В., Погодина Е.С., Юрова Е.В., Белобородов Е.A., Сугак Д.Е., Тумозов И.А., Саенко Ю.В., Фомин А.Н., 20252025Расторгуева Е.В., Погодина Е.С., Юрова Е.В., Белобородов Е.A., Сугак Д.Е., Тумозов И.А., Саенко Ю.В., Фомин А.Н.Rastorgueva E.V., Pogodina E.S., Iurova E.V., Beloborodov E.A., Sugak D.E., Tumozov I.A., Saenko Y.V., Fomin A.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.siboncoj.ru/jour/article/view/3358

Цель исследования – изучить экспрессию C/D мякРНК ((англ. snoRNA) малые ядрышковые РНК) в хромосомах с разным уровнем нарушений и оценить возможность их использования в качестве биомаркеров радиорезистентности при хромосомных аномалиях. Материал и методы. В исследовании сравнивали значения log2FC экспрессии мякРНК C/D семейства в радиочувствительной (HL-60) и радиорезистентной (K562) клеточных линиях, с разным уровнем хромосомных нарушений. Клетки облучались рентгеновским излучением однократно в дозе 4 Гр. Оценивали экспрессию мякРНК C/D через 1, 4 и 24 ч после облучения при помощи секвенирования нового поколения (NGS) MiSeq. Результаты. Получили различные значения log2FC в HL-60 и K562 клеточных линиях через 1, 4 и 24 ч после облучения. В HL-60 преобладала положительная экспрессия C/D мякРНК в ходе всего эксперимента. В К562 через 4 ч после облучения наблюдали преобладание положительных значений экспрессии C/D мякРНК, а через 24 ч – отрицательных значений log2FC. Чем больше встречалось аномалий в хромосоме, тем большую разницу в экспрессии мы наблюдали. При этом количество C/D мякРНК максимально изменялось после облучения через 24 ч в исследуемых клеточных линиях. Нами отмечено большее количество C/D мякРНК в клеточной линии HL-60, и всего 3 экспрессирующиеся C/D мякРНК в 15-й маркерной хромосоме в K562 из 16 в HL-60 в этой же хромосоме. Заключение. Показана слабая информативность использования C/D мякРНК семейства в качестве маркеров радиочувствительности при наличии хромосомных аномалий в лейкозных клетках.

The study objective. This paper reviews the express of C/D box snoRNAs (small nucleolar RNAs) and possibility of their use as biomarkers of radioresistance in chromosomal abnormalities. Material and Methods. The study compared the values of log2FC express of snoRNA C/Dbox in radiosensitive (HL-60) and radioresistant (K562) cell lines with different levels of chromosomal abnormalities. The cells were irradiated with X-ray radiation once at a dose of 4 Gy. The expression of snoRNA C/D was evaluated 1, 4 and 24 hours after irradiation, using new generation sequencing (NGS) MiSeq. Results. Different log2FC values were obtained in HL-60 and K562 cell lines 1 hour, 4 and 24 hours after irradiation. Positive expression of C/D snoRNA prevails in HL-60 throughout the experiment. In K562, the predominance of positive values of C/D snoRNA expression was observed 4 hours after irradiation, and negative values of log2FC were observed 24 hours later. the more anomalies there were in the chromosome, the greater the difference in expression we observed. at the same time, the number of C/D snoRNA changed maximally 24 hours after irradiation in the studied cell lines. We noted a greater number of C/D snoRNAs in the HL-60 cell line, and only 3 expressed C/D snoRNAs in the 15th marker chromosome in K562 out of 16 in HL-60 in the same chromosome. Conclusion. Our study showed a low informative value of using C/D snoRNAs family as markers of radiosensitivity in the presence of chromosomal abnormalities in cancer cells.

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The authors declare that there are no conflicts of interest present.