oncotomskСибирский онкологический журналSiberian journal of oncology1814-48612312-3168Tomsk National Research Medical Сепtеr of the Russian Academy of Sciences10.21294/1814-4861-2025-24-3-76-92oncotomsk-3666Research ArticleЛАБОРАТОРНЫЕ И ЭКСПЕРИМЕНТАЛЬНЫЕ ИССЛЕДОВАНИЯLABORATORY AND EXPERIMENTAL STUDIESАссоциация полиморфизмов гена Интерлейкина-10 (rs1800872 и rs1800896) с восприимчивостью к раку шейки матки в Кветте, ПакистанAssociation of Interleukin-10 gene polymorphisms (rs1800872 and rs1800896) with cervical cancer susceptibility in Quetta, Pakistanhttps://orcid.org/0009-0007-0430-5126MohammadN.MohammadN.

Mohammad Nadia - M. Phil., научный сотрудник.

87300, Кветта, Brewery Road

Mohammad Nadia - M. Phil., Researcher.

Quetta, 87300

https://orcid.org/0009-0002-4117-8780KhanM.KhanM.

Khan Maria - M. Phil., научный сотрудник.

87300, Кветта, Brewery Road

Khan Maria - M. Phil., Researcher.

Quetta, 87300

https://orcid.org/0000-0001-6870-2904MaqsoodМ.MaqsoodM.

Maqsood Maryam - PhD (медицинская микробиология), доцент.

87300, Кветта, Brewery Road

Maqsood Maryam - PhD (Medical Microbiology), Assistant Professor.

Quetta, 87300

maryam.umar1@yahoo.comhttps://orcid.org/0009-0002-3543-0425NaseebA.H.K.NaseebA.H.K.

Hafiz Khush Naseeb Ahmed - MBBS, FCPS, радиолог, директор.

Кветта

87300, Кветта, Brewery Road

Hafiz Khush Naseeb Ahmed - MBBS, FCPS, Nuclear Medicine Physician, Director.

Brewery Road, Quetta, 87300

Кафедра зоологии, факультет естественных наук, Женский университет им. Сардара Бахадура ХанаПакистанDepartment of Zoology, Faculty of Life Sciences, Sardar Bahadur Khan Women’s UniversityPakistanЦентр ядерной медицины и радиотерапии (CENAR)ПакистанCenter for Nuclear Medicine and Radiotherapy (CENAR)Pakistan2025040820252437692Copyright © Mohammad N., Khan M., Maqsood М., Naseeb A., 20252025Mohammad N., Khan M., Maqsood М., Naseeb A.Mohammad N., Khan M., Maqsood M., Naseeb A.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.siboncoj.ru/jour/article/view/3666Актуальность

Актуальность. Интерлейкин-10 (IL-10) играет важную роль в регуляции иммунных реакций и может быть связан с развитием рака. Полиморфизмы в гене ИЛ-10, такие как rs1800872 и rs1800896, могут влиять на восприимчивость к раку. Ни одно из предыдущих исследований не изучало связь между этими вариантами и раком шейки матки в Кветте, Пакистан.

Целью исследования было изучение связи между полиморфизмами гена IL-10 (rs1800872 и rs1800896) и восприимчивостью к раку шейки матки (РШМ) среди женщин в Кветте, Пакистан, а также определение распространенности и факторов риска, способствующих развитию РШМ в этой группе населения.

Материал и методы

Материал и методы. Для проведения анализа по методу «случай-контроль» были отобраны 50 больных РШМ и 50 здоровых лиц. Данные для ретроспективного анализа собирались с использованием заранее разработанной формы. Демографическая информация и образцы крови собирались у участниц с их согласия. ДНК извлекалась с использованием органического подхода, а генотипирование проводилось с использованием техники TETRA-праймера ARMS-PCR. Анализ данных проводился с использованием мультиномиальной логистической регрессии с 95 % доверительным интервалом с помощью программного обеспечения SPSS.

Результаты

Результаты. Исследование не выявило значимой связи между полиморфизмами гена IL-10 (rs1800872 и rs1800896) и РШМ среди населения города Кветта. Значимая связь была обнаружена между РШМ и курением, отсутствием физической активности, возрастом менархе и использованием оральных контрацептивов.

Заключение

Заключение. Исследование не подтверждает связи между полиморфизмами гена IL-10 (rs1800872 и rs1800896) и раком шейки матки в Кветте. Отсутствие осведомленности и неадекватный скрининг являются существенным препятствием для эффективного лечения РШМ.

Background

Background. Interleukin-10 (IL-10) regulates immune responses and has been linked to cancer development. Polymorphisms in the IL-10 gene, such as rs1800872 and rs1800896, may affect cancer susceptibility. No previous study has examined the association between these variants and cervical cancer in Quetta, Pakistan, which this study aims to address.

Aims

Aims. This study aimed to investigate the association between IL-10 gene polymorphisms (rs1800872 and rs1800896) and cervical cancer susceptibility among women in Quetta, Pakistan, and to determine the prevalence and risk factors contributing to cervical cancer in this population.

Material and Methods

Material and Methods. A total of 50 patients diagnosed with cervical cancer and 50 individuals without any health issues were selected for the case-control analysis. Data was collected for retrospective analysis using a pre-designed data collection form. Demographic information and blood samples were collected from participants with explicit consent. The DNA was extracted using an organic approach, and genotyping was performed using the TETRA primer ARMS-PCR technique. The data analysis was conducted using multinomial logistic regression with a 95 % confidence interval, utilizing the SPSS software.

Results

Results. The study demonstrated no significant association between IL-10 gene polymorphisms (rs1800872 and rs1800896) and cervical cancer among the population of Quetta City. Statistically significant relationships were found between cervical cancer and smoking, lack of exercise, menarche, and usage of oral contraceptive medications.

Conclusion

Conclusion. This study confirms no association between IL-10 gene polymorphisms (rs1800872 and rs1800896) and cervical cancer in Quetta. Furthermore, a lack of awareness regarding cervical cancer poses a significant obstacle to its effective care at the individual level in Pakistan.

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The authors declare that there are no conflicts of interest present.