AN IN VITRO ESTIMATION OF CYTOTOXICITY OF FUSARIUM TRICHOTHECENE ON THE BREAST CANCER CELL LINE
https://doi.org/10.21294/1814-4861-2019-18-6-90-95
Abstract
trichothecenes and their derivatives have recently attracted much attention of researchers with respect of their potential role in medicine, including for the treatment of different types of cancer. The purpose of the study was to investigate the cytotoxic effect of Fusarium trichothecene on human breast cancer cells, human skin fibroblasts and embryonic kidney cells in vitro. Material and methods. Based on the Mtt assay, the cytotoxic effect of trichothecene on cell cultures was determined. Evaluation of morphological changes in cell cultures under the influence of trichothecene was performed by light microscopy. Results. Fusarium trichothecene was found to exhibit a dose-dependent toxic effect on cell lines in the range 1 nM to 1000 nM. the most pronounced cytotoxic effect of trichothecene was observed in human breast cancer cell line (IС50 94.72 ± 4.1 нМ). Lower doses of trichothecene led to a change in the size, shape of human breast cancer cells, human skin fibroblasts and embryonic kidney cells, and loss of contact between them and their isolation. the degradation of cell membranes, formation of unformed cell aggregates and fragments were observed in higher doses of trichothecene. Conclusion. Fusarium trichothecen is a biologically active compound and is less toxic to the normal than to the cancer cell lines, therefore, further studies of this agent are needed.
About the Authors
Rin. S. Mukhammadiev
Federal center of Toxicological, Radiological and Biological Safety
Russian Federation
Russian Federation
PhD, Researcher of Sector of Tissue Technology and Production of Drugs, Department of Toxicology
2, scientific town street, 420008-Kazan, Russia
Rish. S. Mukhammadiev
Federal center of Toxicological, Radiological and Biological Safety
Russian Federation
Russian Federation
PhD, Researcher of Sector of Tissue Technology and Production of Drugs, Department of Toxicology
2, scientific town street, 420008-Kazan, Russia
V. V. Biryulya
Federal center of Toxicological, Radiological and Biological Safety
Russian Federation
Russian Federation
PhD, Leading Researcher of Sector of Tissue Technology and Production of Drugs, Department of Toxicology
2, scientific town street, 420008-Kazan, Russia
I. I. Idiyatov
Federal center of Toxicological, Radiological and Biological Safety
Russian Federation
Russian Federation
PhD, Senior Researcher of Sector of Tissue Technology and Production of Drugs, Department of Toxicology
2, scientific town street, 420008-Kazan, Russia
A. A. Nabatov
Kazan State Medical Academy
Russian Federation
Russian Federation
DSc, Associate Professor of Department of Biochemistry and Clinical Laboratory Diagnostics
11, Mushtari street, 420012-Kazan, Russia
A. P. Glinushkin
All-Russia Research Institute of Phytopatology
Russian Federation
Russian Federation
DSc, Director
5, Bolshie Vyazemy, 143050-Moscow Region, Russia
L. R. Valiullin
Federal center of Toxicological, Radiological and Biological Safety
Russian Federation
Russian Federation
PhD, Head of Sector of Tissue Technology and Production of Drugs, Department of Toxicology
2, scientific town street, 420008-Kazan, Russia
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For citations:
Mukhammadiev R.S., Mukhammadiev R.S., Biryulya V.V., Idiyatov I.I., Nabatov A.A., Glinushkin A.P., Valiullin L.R. AN IN VITRO ESTIMATION OF CYTOTOXICITY OF FUSARIUM TRICHOTHECENE ON THE BREAST CANCER CELL LINE. Siberian journal of oncology. 2019;18(6):90-95. (In Russ.) https://doi.org/10.21294/1814-4861-2019-18-6-90-95
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