CIRCULATING ACTIN-BINDING PROTEINS IN PROGRESSION OF LARYNGEAL AND HYPOHARYNGEAL CANCERS
https://doi.org/10.21294/1814-4861-2020-19-4-88-93
Abstract
High aggressiveness of laryngeal and hypopharyngeal squamous cell carcinomas dictates the necessity of studying the molecular mechanisms of metastasis. Cancer metastasis is associated with remodeling of the cytoskeleton, which is carried out by actin-binding proteins (ABPs). Currently, there is insufficient data on the feasibility of determining the level of circulating ASBs in lymphogenous metastasis of laryngeal cancer (LC).
Material and Methods. Blood serum analysis was carried out in 42 LC patients and 15 healthy volunteers using ELISA kits on a microplate ELISA reader Multiskan FC 100 (ThermoFisher Scientific). Among ASBs, cofilin1 (CFL1), fascin1 (FSCN1), ezrin (EZR), profilin1 (PFN1), adenylyl cyclase associated protein 1 (CAP1) were studied. Statistical processing of the results was performed using the Statistica 6.0 software package.
Results. It was shown that the serum level of CAP1 was significantly higher in patients with LC compared with the group of healthy volunteers (p=0.00). As the size of the primary tumor increased, the levels of FSCN1, CAP1 and PFN1 significantly increased. The level of FSCN1 was 10 times higher and the level of CAP1 was 40% higher in LC patients with metastases than in LC patients without metastases. Thus, among the studied ASBs, FSCN1, CAP1, and PFN1 play an important role in the pathogenesis of LC.
Conclusion. The results of the serum level of ASB in LC were obtained for the first time, and they determine the fundamental basis for the development of new methods for predicting the development of metastases.
Material and Methods. Blood serum analysis was carried out in 42 LC patients and 15 healthy volunteers using ELISA kits on a microplate ELISA reader Multiskan FC 100 (ThermoFisher Scientific). Among ASBs, cofilin1 (CFL1), fascin1 (FSCN1), ezrin (EZR), profilin1 (PFN1), adenylyl cyclase associated protein 1 (CAP1) were studied. Statistical processing of the results was performed using the Statistica 6.0 software package.
Results. It was shown that the serum level of CAP1 was significantly higher in patients with LC compared with the group of healthy volunteers (p=0.00). As the size of the primary tumor increased, the levels of FSCN1, CAP1 and PFN1 significantly increased. The level of FSCN1 was 10 times higher and the level of CAP1 was 40% higher in LC patients with metastases than in LC patients without metastases. Thus, among the studied ASBs, FSCN1, CAP1, and PFN1 play an important role in the pathogenesis of LC.
Conclusion. The results of the serum level of ASB in LC were obtained for the first time, and they determine the fundamental basis for the development of new methods for predicting the development of metastases.
Keywords
Supporting agencies: This study was financially supported by the Russian Foundation for Basic Research (Grant No. 20-015-00151 A).
About the Authors
G. V. Kakurina
Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences
Russian Federation
Russian Federation
Gelena V. Kakurina, PhD, Senior Researcher, Laboratory of Tumor Biochemistry
Researcher ID (WOS): C-8668-2012. AuthorID (Scopus): 23667534500
5, Kooperativny Street, 634009-Tomsk
E. E. Shashova
Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences
Russian Federation
Russian Federation
Elena E. Shashova, PhD, Senior Researcher, Laboratory of Tumor Biochemistry
Researcher ID (WOS): D-1472-2012. AuthorID (Scopus): 54994115800.
5, Kooperativny Street, 634009-Tomsk
O. V. Cheremisina
Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences
Russian Federation
Russian Federation
Olga V. Cheremisina, MD, DSc, Head of Endoscopy Department
Researcher ID (WOS): C-9259-2012. Author ID (Scopus): 6602197938.
5, Kooperativny Street, 634009-Tomsk
E. L. Choinzonov
Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences
Russian Federation
Russian Federation
Evgeny L. Choynzonov, MD, DSc, Professor, Member of the Russian Academy of Sciences, Director; Head of Oncology Department of Siberian State Medical University.
Researcher ID (WOS): P-1470-2014. Author ID (Scopus): 6603352329.
5, Kooperativny Street, 634009-Tomsk
I. V. Kondakova
Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences
Russian Federation
Russian Federation
Irina V. Kondakova, DSc, Professor, Head of the Laboratory of Tumor Biochemistry
Researcher ID (WOS): C-8658-2012. AuthorID (Scopus): 6701872510.
5, Kooperativny Street, 634009-Tomsk
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Review
For citations:
Kakurina G.V., Shashova E.E., Cheremisina O.V., Choinzonov E.L., Kondakova I.V. CIRCULATING ACTIN-BINDING PROTEINS IN PROGRESSION OF LARYNGEAL AND HYPOHARYNGEAL CANCERS. Siberian journal of oncology. 2020;19(4):88-93. (In Russ.) https://doi.org/10.21294/1814-4861-2020-19-4-88-93
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