Mapping of an epitope of ErbB-2 for recognizing by monoclonal antibody 4B8 using phage display technolog
Abstract
This article presents identification and mapping of an epitope in the protein ErbB-2 which binds to the monoclonal antibody 4B8 intended for use in immunochemistry to estimate the level of ErbB-2 production. Epitope was identified by biopanning of phage display library based on filamentous phage. Location of the epitope was revealed in ClustalW program, analysis of specificity was carried out by ELISA. The epitope is also similar to the sequence on tyrosine kinase domain. It suggests a possible interaction of this site with 4B8.
About the Authors
M. B. Borgoyakova
State Research Center of Virology and Biotechnology VECTOR, Novosibirsk region, Koltsovo, Novosibirsk State University, Novosibirsk
Russian Federation
Russian Federation
A. A. Ilichev
ФБУН ГНЦ ВБ «Вектор», р.п. Кольцово, Новосибирская обл., Новосибирский государственный университет, г. Новосибирск
Russian Federation
Russian Federation
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Review
For citations:
Borgoyakova M.B., Ilichev A.A. Mapping of an epitope of ErbB-2 for recognizing by monoclonal antibody 4B8 using phage display technolog. Siberian journal of oncology. 2013;(4):40-44. (In Russ.)
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