IMMUNOHISTOCHEMICAL STUDY OF MSH2, MSH6, PMS2, MLH1 IN EVALUATION OF DIFFERENTIATION GRADE OF COLON ADENOCARCINOMA
Abstract
Microsatellite instability is associated with dysfunction of the MSH2, MLH1, PMS2 and MSH6 genes, which participate in the repair of unpaired nucleotides of DNA. It is known that microsatellite instability is an independent prognostic factor in determining the differentiation grade of colon cancer. The use of immunohistochemistry to study the repair system of unpaired nucleotides has its own characteristics and limitations. Materials and methods. The study included 39 patients with colon adenocarcinoma. Moderately-differentiated colon adenocarcinoma was the most common histological type (72 %). There were 8 % of well-differentiated and 12 % poorly-differentiated carcinomas. Immunohistochemical analysis of SH2, MSH6, PMS2 and MLH1 proteins was done according to the standard protocol. Results. Out of 39 cases, 6 (15 %) had loss of expression of at least one of the studied proteins. Out of these 6 cases with indirect signs of MSI-H, 3 were poorlydifferentiated, 1 was mucinous and 2 were moderately differentiated adenocarcinomas. Conclusion. Thus, immunohistochemical analysis of DNA repair genes can be used to determine the histological differentiation of colon adenocarcinoma.
About the Authors
G. A. Raskin
Russian Research Center for Radiology and Surgical Technologies, Saint Petersburg;
Saint Petersburg State University
Russian Federation
Russian Federation
Raskin Grigory Alexandrovich, MD, PhD, Leading Researcher.
E-mail: rasking@list.ru. SPIN-code: 4569-9756
S. V. Petrov
Kazan State Medical University
Russian Federation
Russian Federation
Petrov Semyen Venediktovich, MD, Professor, Pathology Department.
SPIN-код: 3237-4735
R. V. Orlova
Russian Research Center for Radiology and Surgical Technologies, Saint Petersburg;
Saint Petersburg State University
Russian Federation
Russian Federation
Orlova Rashida Vakhidovna, MD, Professor, Head of Oncology Department, Medical Faculty, Saint Petersburg State University.
SPIN-code: 9932-617
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Review
For citations:
Raskin G.A., Petrov S.V., Orlova R.V. IMMUNOHISTOCHEMICAL STUDY OF MSH2, MSH6, PMS2, MLH1 IN EVALUATION OF DIFFERENTIATION GRADE OF COLON ADENOCARCINOMA. Siberian journal of oncology. 2015;1(5):80-83. (In Russ.)
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