EXPRESSION PROFILE OF MICRORNAS IN SQUAMOUS CELL HEAD AND NECK CARCINOMA
Abstract
Head and neck squamous cell carcinoma (HNSCC) is a highly aggressive disease with long asymptomatic course. New molecular prognostic markers are urgently needed to identify patients at high risk for developing lymph node metastasis. MicroRNAs (miRNAs) are a recently discovered class of small non-coding RNAs. Based on literature and our data, we have chosen 12 miRNAs (miRNA-21, -221, -222, -155, -205, -20a, -125b, -146b, -181b, -200a, -126, -451), involved in HNSCC carcinogenesis. Using real-time quantitative polymerase chain reaction analysis, we have shown a change in the expression miRNA in tumor tissue compared to unmodified tissue. Significant upregulation of five miRNAs: miRNA-21, -155, -181b, -126, -451 (p<0.05) has been shown in tumors. MiRNA-126 has been found to be highly expressed in metastasis tissue (p<0.05), and can be an important factor in understanding the processes of metastasis and development of a new prognostic marker.
About the Authors
Yu. A. Veryaskina
Institute of Molecular and Cellular Biology SB RAS, Novosibirsk
Russian Federation
Russian Federation
Veryaskina Yuliya Andreevna, senior assistant of the Laboratory of Molecular Genetics.
E-mail: fl-31@mail.ru. SPIN-code: 7989-6099
G. V. Kakurina
Tomsk Cancer Research Institute
Russian Federation
Russian Federation
Kakurina Gelena Valerevna, MD, Ph.D., Senior Researcher of the Laboratory of Tumor Biochemistry.
E-mail: kakurinagv@oncology.tomsk.ru. SPIN-code: 1896-3144.
E. S. Zhuravlev
Institute of Molecular and Cellular Biology SB RAS, Novosibirsk
Russian Federation
Russian Federation
Zhuravlev Evgeniy Sergeevich, senior assistant of the Laboratory of Molecular Genetics.
E-mail: evgenijur@gmail.com. SPIN-code: 6760-3609.
S. E. Titov
Institute of Molecular and Cellular Biology SB RAS, Novosibirsk;
Vector-Best Ltd, Novosibirsk
Russian Federation
Russian Federation
Titov Sergey Evgenevich, Ph.D., Researcher of Ltd «Vector-Best», Novosibirsk, senior assistant of the Laboratory of Molecular Genetics, Institute of Molecular and Cell Biology SB RAS (Novosibirsk).
E-mail: titovse78@gmail.com. SPIN-code: 4924-8365.
I. V. Kondakova
Tomsk Cancer Research Institute
Russian Federation
Russian Federation
Kondakova Irina Viktorovna, Tumor Biochemistry.
E-mail: kondakova@oncology.tomsk.ru. SPIN-code: 9338-4149.
O. V. Cheremisina
Tomsk Cancer Research Institute
Russian Federation
Russian Federation
Cheremisina Olga Vladimirovna, MD, DSc, Head of Endoscopy Department.
E-mail: СheremisinaOV@oncology.tomsk.ru. SPIN-code: 9579-2691.
D. A. Shishkin
Tomsk Cancer Research Institute
Russian Federation
Russian Federation
Shishkin Dmitriy Aleksandrovich, Ph.D., Senior Researcher of the laboratory of the department of head and neck tumors.
E-mail: nii@oncology.tomsk.ru. SPIN-code: 5793-2432.
I. F. Zhimulev
Institute of Molecular and Cellular Biology SB RAS, Novosibirsk
Russian Federation
Russian Federation
Zhimulev Igor Fedorovich, DSc, Professor, Academician RAN, Director of Institute.
Phone: 8 (383) 363-90-41. E-mail: zhimulev@mcb.nsc.ru. SPIN-code: 5924-5307.
E. L. Choynzonov
Tomsk Cancer Research Institute;
Siberian State Medical University, Tomsk
Russian Federation
Russian Federation
Choinzonov Evgeny Lhamatsirenovich, MD, DSc, Professor, Academician of RAS, Director of the Tomsk Cancer Research Institute.
E-mail: nii@oncology.tomsk.ru. SPIN-code: 2240-8730.
N. N. Kolesnikov
Institute of Molecular and Cellular Biology SB RAS, Novosibirsk
Russian Federation
Russian Federation
Kolesnikov Nikolay Nikolaevich, DSc, Principal Investigator of the laboratory of molecular genetics.
E-mail: kolesnikovnn@mcb.nsc.ru. SPIN-code: 6236-1344.
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Review
For citations:
Veryaskina Yu.A., Kakurina G.V., Zhuravlev E.S., Titov S.E., Kondakova I.V., Cheremisina O.V., Shishkin D.A., Zhimulev I.F., Choynzonov E.L., Kolesnikov N.N. EXPRESSION PROFILE OF MICRORNAS IN SQUAMOUS CELL HEAD AND NECK CARCINOMA. Siberian journal of oncology. 2015;(6):33-38. (In Russ.)
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