Chymotrypsin-like and caspase-like proteasome activities in breast cancer
Abstract
The chymotrypsin-like and caspase-like proteasome activities were studied in tumor tissues of 74 patients with breast cancer (BC) NМin association with tumor size and lymph node involvement. Chymotrypsin-like and caspase-like proteasome activities were Т1-40-30 higher in breast cancer tissue than in adjacent tissues. We showed that advanced BC is accompanied by increase in chymotrypsin-like and caspase-like proteasome activities. In tumors with lymph node dissemination, proteasome activity was significantly decreased, which may have prospective prognostic value. Taken together, our data indicate an important role of intracellular proteolysis in BC progression.
About the Authors
E. E. Shashova
Cancer Research Institute, SB RAMS, Tomsk
Russian Federation
Russian Federation
I. V. Kondakova
Cancer Research Institute, SB RAMS, Tomsk
Russian Federation
Russian Federation
E. M. Slonimskaya
Cancer Research Institute, SB RAMS, Tomsk, Siberian State Medical University, Tomsk
Russian Federation
Russian Federation
S. A. Glushenko
Cancer Research Institute, SB RAMS, Tomsk
Russian Federation
Russian Federation
E. S. Kolegova
Siberian State Medical University, Tomsk
Russian Federation
Russian Federation
References
1. Кондакова И. В., Спирина Л. В., Шашова Е. Е. и др. Активность протеасом в опухолях женской репродуктивной системы//Биоорганическая химия. 2012. Т. 38, № 1. С. 106-110
2. Середа Е. Е., Кондакова И. В., Слонимская Е. М. Ферменты метаболизма эстрогенов и рецепторы как факторы риска развития и прогноза при раке молочной железы//Сибирский онкологический журнал. 2004. № 1 (9). С. 35-43
3. Спирина Л.В., Кондакова И.В., Усынин Е.А. и др. Активность протеасом в тканях злокачественных опухолей различных локализаций//Сибирский онкологический журнал. 2009. № 5 (35). С.49-52
4. Спирина Л.В., Кондакова И.В., Усынин Е.А. и др. Регуляция экспрессии транскрипционных факторов и фактора роста эндотелия протеасомной системой при метастазировании рака почки//Вестник РОНЦ им. Н.Н. Блохина РАМН. 2012. Т. 23, № 1. С. 27-32
5. Цимоха А.С. Протеасомы: участие в клеточных процессах//Цитология. 2010. Т. 52, № 4. С. 277-300
6. Чойнзонов Е. Л., Спирина Л. В., Кондакова И. В. и др. Роль внутриклеточныхпротеиназврегуляцииэкспрессиитранскрипционных факторов HIF-1, NF-kB и фактора роста сосудов при лимфогенном метастазировании плоскоклеточных карцином головы и шеи//Бюллетень СО РАМН. 2012. № 6. С. 15-21
7. Шашова Е.Е., Кондакова И.В., Слонимская Е.М. и др. Сравнительное изучение содержания рецепторов эстрогенов и прогестерона в неизмененной, опухолевой и метастатической тканях при раке молочной железы//Сибирский онкологический журнал. 2008. № 4 (28). С. 42-45
8. Ben-Shahar S., Komlosh A., Nadav E. et al. 26 S proteasome-mediated production of an authentic major histocompatibility class I-restricted epitope from an intact protein substrate//J. Biol. Chem. 1999. Vol. 274 (31). P. 21963-21972
9. Ha N.-H, Nair V.S., Reddy D.N. et al. Lactoferrin-endothelin-1 axis contributes to the development and invasiveness of triple-negative breast cancer phenotypes//Cancer Res. 2011. Vol.71. P. 7259-7269
10. Jones M.D., Liu J.C., Thomas K. A Proteasome Inhibitor, Bortezomib, Inhibits Breast Cancer Growth and Reduces Osteolysis by Downregulating Metastatic Genes//Clin. Cancer Res. 2010. Vol. 16. P. 4978-4989
11. Kretzer N.M., Cherian M.T., Mao C. et al. A Noncompetitive small molecule inhibitor of estrogen-regulated gene expression and breast cancer cell growth that enhances proteasome-dependent degradation of estrogen Receptor-α//J. Biol. Chem. 2010. Vol. 285 (53). P. 41863-41873
12. Kisselev A., Callard A., Goldberg A. Importance of the different proteolytic sites of the proteasome and the efficacy of inhibitors varies with the protein substrate//J. Biol. Chem. 2006. Vol. 281 (13). P. 8582-8590
13. Landis-Piwowar K.R., Milacic V., Chen D. et al. The proteasome as a potential target for novel anticancer drugs and chemosensitizers//Drug Resist. Updat. 2006. Vol. 9. P. 263-273
14. Lie C., Kiran M. Increased proteasome activity, ubiquitin-conjugating enzymes, and eEF1A translation factor detected in breast cancer tissue//Cancer Res. 2005. Vol. 65 (13). P. 5599-5606
15. Li C., Li R., Grandis J.R., Johnson D.E. Bortezomib induces apoptosis via Bim and Bik up-regulation and synergizes with cisplatin in the killing of head and neck squamous cell carcinoma cells//Mol. Cancer Ther. 2008. Vol. 7 (6). P. 1647-1655
16. Lowry O.H., Rosenbrough N.J., Randall R.J. Protein measurement with the folin phenol reagent//J. Biol. Chem. 1951. Vol. 193. Р. 265-275
17. La Rosa P., Pesiri V., Leclercq G. et al. Palmitoylation regulates 17-estradiol-induced estrogen receptor-degradation and transcriptional activity//Mol. Endocrinol. 2012. Vol. 26. P. 762-764
18. Marx C., Yau C., Banwait S. et al. Proteasome-Regulated ERBB2 and Estrogen Receptor Pathways in Breast Cancer//Mol. Pharmacol. 2007. Vol. 71. P. 1525-1534
19. Milano A., Iaffaioli R.V., Caponigro F. The proteasome: a worth while target for the treatment of solid tumours?//Eur. J. Cancer. 2007. Vol 43. P. 1125-1133
20. Spirina L.V., Yunusova N.V., Kondakova I.V. et al. Association of growth factors, HIF-1 and NF-κB expression with proteasomes in endometrial cancer//Mol. Biol. Repor. 2012. Vol. 39 (9). P. 8655-86
21. Spirina L.V., Kondakova I.V., Choynzonov E.L. et al. Expression of vascular endothelial growth factor and transcription factors HIF-1, NF-kB expression in squamous cell carcinoma of head and neck; association with proteasome and calpain activities//J. Cancer Res. Clin. Oncol. 2013. Vol. 139. P. 625-633
22. Sharova N.P., Astakhova T.M., Karpova Y.D. et al. Changes in proteasome pool in human papillary thyroid carcinoma development//Centr. Eur. J. Biol. 2011. Vol. 6 (4). P. 486-496
23. Xie Y. Structure, Assembly and Homeostatic Regulation of the 26S Proteasome//J. Mol. Cell Biol. 2010. Vol. 2 (6). P. 308-317
24. Xu H., Ju D., Jarois T., Xie Y. Diminished feedback regulation of proteasome expression and resistance to proteasome inhibitors in breast cancer cells//Breast Cancer Res. Treat. 2008. Vol. 107. P. 267-274
Review
For citations:
Shashova E.E., Kondakova I.V., Slonimskaya E.M., Glushenko S.A., Kolegova E.S. Chymotrypsin-like and caspase-like proteasome activities in breast cancer. Siberian journal of oncology. 2013;(5):45-49. (In Russ.)
Views:
494
Email this article 
