NEW MONOCLONAL ANTIBODY FOR TUMOR-ASSOCIATED PROTEIN PMEPA1 DETECTION
Abstract
We obtained a new monoclonal antibody (mAb) against tumor-associated protein PMEPA1 (Prostate Transmembrane Protein, Androgen Induced 1) for which is shown differential expression in some tumors. To test the specificity of obtained mAb we have produced a full-length recombinant PMEPA1 in transfected HEK293T cell line. After transfection of the plasmid vector pIRES-EGFP, containing the insertion coding PMEPA1 gene we evaluated PMEPA1 protein expression in HEK293T cells lines by immunocytochemical study and western-blot analysis. Ability of the new mAb binds PMEPA1 in clinical specimens is shown by immunohistochemistry on histological sections of prostate tissue used as positive control. New antibody may provide a basis for development of commercial antibody test for evaluation of protein expression in human biological samples.
About the Authors
V. V. Volkomorov
Cancer Research Institute of Siberian Вranch of the Russian Academy of Medical Sciences, Tomsk
National Research Tomsk State University, Tomsk
Russian Federation
Russian Federation
E. S. Grigoryeva
Cancer Research Institute of Siberian Вranch of the Russian Academy of Medical Sciences, Tomsk
National Research Tomsk State University, Tomsk
Russian Federation
Russian Federation
M. S. Karbyshev
Siberian State Medical University, Tomsk
Russian Federation
Russian Federation
I. V. Mitrofanofa
International Biotechnology Center «Generium», Petushinski district, Volginsky village
Russian Federation
5, Kooperativny Street, 634050-Tomsk
Russian Federation
5, Kooperativny Street, 634050-Tomsk
E. V. Kaigorodova
Cancer Research Institute of Siberian Вranch of the Russian Academy of Medical Sciences, Tomsk
National Research Tomsk State University, Tomsk
Russian Federation
Russian Federation
N. V. Cherdyntseva
Cancer Research Institute of Siberian Вranch of the Russian Academy of Medical Sciences, Tomsk
National Research Tomsk State University, Tomsk
Russian Federation
Russian Federation
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Review
For citations:
Volkomorov V.V., Grigoryeva E.S., Karbyshev M.S., Mitrofanofa I.V., Kaigorodova E.V., Cherdyntseva N.V. NEW MONOCLONAL ANTIBODY FOR TUMOR-ASSOCIATED PROTEIN PMEPA1 DETECTION. Siberian journal of oncology. 2014;(3):72-77. (In Russ.)
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