ELLIPSOMETRY, RAMAN SPECTROSCOPY AND SOI- NANOWIRE BIOSENSOR IN DIAGNOSIS OF COLORECTALCANCER
https://doi.org/10.21294/1814-4861-2017-16-4-32-41
Abstract
Differences in the rate constants of specific interactions between serum tumor M2-pyruvate kinase (Tumor M2-PK) and highly specific monoclonal antibodies deposited on the surface of biochips were found in colorectal cancer patients using surface plasmon resonance enhanced ellipsometry. Scanning ellipsometry revealed a significant increase in the biomolecular layer thickness caused by antigen-antibody reaction in patients with hepatic and extra-hepatic metastases compared to that in healthy subjects (p<0.001–0.042).The specificity of the interaction was confirmed by fluorescence optical spectrometry. The Raman spectra of serum samples revealed differences in the intensity of peaks appeared at 1005–1520 cm-1 in the same groups of patients (p<0.0001–0.05) with a predictive accuracy of 90 % for early-stage disease. The pilot experiments with a nanowire biosensor based on SOI (silicon on insulator), for example Tumor M2-PK, were carried out. High sensitivity (10-13–10-15M) and specificity in identifying antigens in serum samples of patients with colorectal cancer were demonstrated. The results obtained were useful for detecting early-stage disease, metastases and recurrence as well as for monitoring the quality of treatment in colorectal cancer patients.
About the Authors
M. V. Kruchinina
Research Institute of Internal and Preventive Medicine ‑ Branch of the Institute of Cytology and Genetics; Siberian Scientific Research Institute for Metrology, ROSSTANDART.
Russian Federation
Russian Federation
Kruchinina Margarita V., Professor, Leading Researcher, Institution of Internal and Preventive Medicine.
175/1, B. Bogatkogo Str., 630089-Novosibirsk, Russia.
Ya. I. Prudnikova
Research Institute of Internal and Preventive Medicine ‑ Branch of the Institute of Cytology and Genetics.
Russian Federation
Russian Federation
Prudnikova Yana I., Post Graduate, Institution of Internal and Preventive Medicine.
175/1, B. Bogatkogo Str., 630089-Novosibirsk, Russia.
S. A. Kurilovich
Research Institute of Internal and Preventive Medicine ‑ Branch of the Institute of Cytology and Genetics.
Russian Federation
Russian Federation
Kurilovich Svetlana A., Professor, Head of Laboratory, Institution of Internal and Preventive Medicine.
175/1, B. Bogatkogo Str., 630089-Novosibirsk, Russia.
A. A. Gromov
Research Institute of Internal and Preventive Medicine ‑ Branch of the Institute of Cytology and Genetics.
Russian Federation
Russian Federation
Gromov AndreyA., PhD, Head of Laboratory, Institution of Internal and Preventive Medicine.
175/1, B. Bogatkogo Str., 630089-Novosibirsk, Russia.
V. N. Kruchinin
Rzhanov Institute of Semiconductor Physics, SB RAS.
Russian Federation
Kruchinin Vladimir N., PhD, Senior Scientist, Rzhanov Institute of Semiconductor Physics, SB RAS. 13, Academician Lavrentiev Prospect, 630090-Novosibirsk, Russia.
Russian Federation
Kruchinin Vladimir N., PhD, Senior Scientist, Rzhanov Institute of Semiconductor Physics, SB RAS. 13, Academician Lavrentiev Prospect, 630090-Novosibirsk, Russia.
V. V. Atuchin
Rzhanov Institute of Semiconductor Physics, SB RAS; Novosibirsk State University, Novosibirsk.
Russian Federation
Russian Federation
Atuchin Viktor V., PhD, Head of Laboratory, Rzhanov Institute of Semiconductor Physics, SB RAS.
2, Pirogova Str., 630090-Novosibirsk, Russia.
O. V. Naumova
Rzhanov Institute of Semiconductor Physics, SB RAS.
Russian Federation
Russian Federation
Naumova Olga V., Professor, Head of Laboratory, Rzhanov Institute of Semiconductor Physics, SB RAS.
13, Academician Lavrentiev Prospect, 630090-Novosibirsk, Russia.
E. V. Spesivtsev
Rzhanov Institute of Semiconductor Physics, SB RAS.
Russian Federation
Russian Federation
Spesivtsev Evgeny V., PhD, Senior Scientist, Rzhanov Institute of Semiconductor Physics, SB RAS.
13, Academician Lavrentiev Prospect, 630090-Novosibirsk, Russia.
V. A. Volodin
Rzhanov Institute of Semiconductor Physics, SB RAS.
Russian Federation
Russian Federation
Volodin Vladimir A., Professor, Leading Researcher, Rzhanov Institute of Semiconductor Physics, SB RAS.
13, Academician Lavrentiev Prospect, 630090-Novosibirsk, Russia.
S. E. Peltek
Institute of Cytology and Genetics, SB RAS.
Russian Federation
Russian Federation
Peltek Sergey E., PhD, Head of Laboratory, Institute of Cytology and Genetics, SB RAS.
10, Academician Lavrentiev Prospect, 630090-Novosibirsk, Russia.
G. V. Shuvalov
Siberian Scientific Research Institute for Metrology, ROSSTANDART.
Russian Federation
Russian Federation
Shuvalov GennadiyV., PhD, Director of Institute, Siberian Scientific Research Institute for Metrology, ROSSTANDART.
4, Dimitrova Prospect, 630004-Novosibirsk, Russia.
V. M. Generalov
State Research Center of Virology and Biotechnology «VECTOR».
Russian Federation
Russian Federation
Generalov Vladimir M., Professor, Leading Researcher, State Research Center of Virology and Biotechnology «VECTOR».
Koltsovo-630559, Novosibirsk region, Russia.
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Review
For citations:
Kruchinina M.V., Prudnikova Ya.I., Kurilovich S.A., Gromov A.A., Kruchinin V.N., Atuchin V.V., Naumova O.V., Spesivtsev E.V., Volodin V.A., Peltek S.E., Shuvalov G.V., Generalov V.M. ELLIPSOMETRY, RAMAN SPECTROSCOPY AND SOI- NANOWIRE BIOSENSOR IN DIAGNOSIS OF COLORECTALCANCER. Siberian journal of oncology. 2017;16(4):32-41. (In Russ.) https://doi.org/10.21294/1814-4861-2017-16-4-32-41
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