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BREAST CANCER TYPING USING RT-PCR ASSAY

https://doi.org/10.21294/1814-4861-2019-18-5-61-67

Abstract

 Introduction. Adjuvant systemic therapy remains one of the main options for treating breast cancer. Results of standard immunohistochemical studies are not always a criterion for selecting systemic therapy. Nowadays, multigene expression analysis is actively used to predict the response to chemotherapy in patients with earlystage breast cancer. We studied a 24-gene multi-gene panel for typing breast cancer.

Material and Methods. A prospective analysis of 199 breast cancer patients (T1–3N0–3M0) was carried out. Surgical specimens were studied using the standard immunohistochemistry (IHC) and RT-PCR for detecting expression of 24 genes.

Results. According to the IHC results, breast cancer was divided into 5 molecular subtypes: luminal A was detected in 59 (30 %) patients; luminal B (HER2-negative) in 52 (26 %); luminal B (HER2-positive) in 19 (9 %); triple-negative in 28 (14 %); HER2-positive 41 (21 %). RT-PCR showed that ST K15, MYC, MYBL2, BIRCC 5, BCL2, TERT, ESRP1, PGR, HER2, GBR7, MGB1 and MMP11 were the most significant genes in subtype distribution. The total percentage of matches between the two studies was 61.7 %.

Conclusion. Studies have shown the need to add additional typing methods for breast cancer to a standard IHC study, which will undoubtedly increase the information content of diagnostic measures and will improve the effectiveness of the treatment.

About the Authors

V. K. Bozhenko
Russian Scientific Center of Roentgenoradiology of the Ministry of Healthcare of the Russian Federation
Russian Federation

Vladimir K. Bozhenko, MD, Dsc, Professor, Head of the Department of molecular biology and experimental therapy of tumors

86, Profsoyuznaya Street, 117997-Moscow



I. D. Trotsenko
Russian People’s Friendship University (RUDN University)
Russian Federation

Ivan D. Trocenko, MD, PhD, Assistant of the Department of Oncology and X-ray Radiology

6, Mikluho-Maklaya Street, 117198-Moscow



E. A. Kudinova
Russian Scientific Center of Roentgenoradiology of the Ministry of Healthcare of the Russian Federation
Russian Federation

Elena A. Kudinova, MD, Dsc, Head of clinical diagnostic laboratory

86, Profsoyuznaya Street, 117997-Moscow



S. G. Vardanyan
Russian Scientific Center of Roentgenoradiology of the Ministry of Healthcare of the Russian Federation
Russian Federation

Sergei G. Vardanyan, Post-graduate Student

86, Profsoyuznaya Street, 117997-Moscow



M. V. Zakharenko
Russian Scientific Center of Roentgenoradiology of the Ministry of Healthcare of the Russian Federation
Russian Federation

Margarita V. Zaharenko, Junior Researcher of Laboratory of Immunology, Oncocytology and Cell Therapy

86, Profsoyuznaya Street, 117997-Moscow



V. A. Solodky
Russian Scientific Center of Roentgenoradiology of the Ministry of Healthcare of the Russian Federation
Russian Federation

Vladimir A. Solodkij, MD, DSc, Professor, Academician of the Russian Academy of Sciences, Director

86, Profsoyuznaya Street, 117997-Moscow



M. V. Makarova
Russian People’s Friendship University (RUDN University)
Russian Federation

Maria V. Makarova, Post-graduate Student

6, Mikluho-Maklaya Street, 117198-Moscow



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Review

For citations:


Bozhenko V.K., Trotsenko I.D., Kudinova E.A., Vardanyan S.G., Zakharenko M.V., Solodky V.A., Makarova M.V. BREAST CANCER TYPING USING RT-PCR ASSAY. Siberian journal of oncology. 2019;18(5):61-67. (In Russ.) https://doi.org/10.21294/1814-4861-2019-18-5-61-67

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ISSN 1814-4861 (Print)
ISSN 2312-3168 (Online)