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INFLUENCE OF CHRONIC NEUROGENIC PAIN ON LOCAL LEVELS OF SEX HORMONES AND RECEPTORS IN SKIN, TUMOR AND PERIFOCAL AREA IN MALE MICE WITH TRANSPLANTABLE В16/F10 MELANOMA

https://doi.org/10.21294/1814-4861-2020-19-1-73-81

Abstract

Local synthesis and metabolism of sex hormones allow the skin to regulate autonomously both nociceptive reactions and proliferative processes in various pathologies.

The purpose of the study was to reveal the influence of chronic neurogenic pain on the balance of sex hormones in the skin, tumor and perifocal area in male mice in the dynamics of В16/F10 melanoma growth.

Material and methods. The study included С57ВL/6 male mice. Animals were divided into the main group – animals with В16/F10 melanoma transplanted after the development of chronic neurogenic pain (n=25), comparison group (n=27) – animals with standard tumor transplantation, controls – males with chronic neurogenic pain (n=8), and intact mice (n=8). Levels of estradiol (E2), estrone (E1), testosterone (T), progesterone (P4) (CUSABIO, China), estrogen receptors – ERα, ERβ (Сloud-Clone Corp. USA, China), progesterone (RP4) and androgens (RA) (CUSABIO, China) were determined in 10 % homogenates of skin, tumor and perifocal tissues 1, 2 and 3 weeks after В16/F10 melanoma transplantation using standard ELISA test systems adapted for experimental animals.

Results. Chronic neurogenic pain causes an imbalance of sex steroids with marked hyperestrogenism. The imbalance is achieved by increased content of estradiol and receptors of estrogen, increased concentration of progesterone with inhibition of its receptor apparatus, as well as reduced levels of testosterone with increased concentration of androgen receptors. Melanoma in animals with chronic neurogenic pain grows and develops in a target organ with an altered steroid balance, which leads to changes in the tumor characteristics. Chronic neurogenic pain and B16/F10 melanoma has a unidirectional effect on the local hormonal status of the skin.

About the Authors

E. M. Frantsiyants
Rostov Research Institute of Oncology
Russian Federation

DSc, Professor, Deputy General Director for Science, Head of Laboratory of Malignant Tumor Pathogenesis Study

63, 14 Liniya Street, 344037, Rostov-on-Don, Russia




V. A. Bandovkina
Rostov Research Institute of Oncology
Russian Federation

PhD, Senior Researcher, Laboratory of Malignant Tumor Pathogenesis Study

63, 14 Liniya Street, 344037, Rostov-on-Don, Russia




I. M. Kotieva
Rostov Research Institute of Oncology
Russian Federation
MD, PhD, Researcher, Laboratory of Malignant Tumor Pathogenesis Study

63, 14 Liniya Street, 344037, Rostov-on-Don, Russia



I. V. Kaplieva
Rostov Research Institute of Oncology
Russian Federation

MD, PhD, Senior Researcher, Laboratory of Malignant Tumor Pathogenesis Study

63, 14 Liniya Street, 344037, Rostov-on-Don, Russia





L. K. Trepitaki
Rostov Research Institute of Oncology
Russian Federation
Researcher, Laboratory of Malignant Tumor Pathogenesis Study

63, 14 Liniya Street, 344037, Rostov-on-Don, Russia



I. V. Neskubina
Rostov Research Institute of Oncology
Russian Federation
PhD, Senior Researcher, Laboratory of Malignant Tumor Pathogenesis Study

63, 14 Liniya Street, 344037, Rostov-on-Don, Russia



E. I. Surikova
Rostov Research Institute of Oncology
Russian Federation
PhD, Senior Researcher, Laboratory of Malignant Tumor Pathogenesis Study

63, 14 Liniya Street, 344037, Rostov-on-Don, Russia



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Review

For citations:


Frantsiyants E.M., Bandovkina V.A., Kotieva I.M., Kaplieva I.V., Trepitaki L.K., Neskubina I.V., Surikova E.I. INFLUENCE OF CHRONIC NEUROGENIC PAIN ON LOCAL LEVELS OF SEX HORMONES AND RECEPTORS IN SKIN, TUMOR AND PERIFOCAL AREA IN MALE MICE WITH TRANSPLANTABLE В16/F10 MELANOMA. Siberian journal of oncology. 2020;19(1):73-81. (In Russ.) https://doi.org/10.21294/1814-4861-2020-19-1-73-81

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ISSN 1814-4861 (Print)
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