GENE EXPRESSION PROFILE AND MOLECULAR-GENETIC ANALYSIS OF SYNOVIAL SARCOMA AND EWING’ SARCOMA/PNET
Abstract
The relationship between histological and immunohistochemical parameters of synovial sarcoma and Ewing’s sarcoma/PNET and the number of cells carrying the t(X;18)(p11.2;q11.2) translocation. Histological examination was carried out to study the cytotypical characteristics, types of structures of tumor growth, tumor spread and secondary dystrophic changes in the tumor. The tumor mitotic activity was assessed by determining the mitotic index. The presence of markers such as vimentine, desmin, SMA, Myf-4, MyoD1, S-100, CD57, bcl-2, CD99, cytokeratine AE1/AE3, cytokeratine 7, EMA, Synaptophysin, chromogranin and Ki67 was determined by immunohistochemical assay. Detection of translocations characteristic of synovial sarcoma and Ewing’s sarcoma/PNET was performed using chromogenic in situ hybridization. The study results showed the relationship between the histological and immunohistochemical parameters of synovial sarcoma and Ewing’s sarcoma/PNET and the percentage of tumor cells having specific t(X;18)(p11.2;q11.2) translocation.
About the Authors
V. М. Perelmuter
Cancer Research Institute, Siberian Branch of the Russian Academy of Medical Sciences, Tomsk
Siberian State Medical University, Tomsk
Russian Federation
5, Kooperativny Street, Tomsk-634050
Russian Federation
5, Kooperativny Street, Tomsk-634050
N. V. Vasilyev
Cancer Research Institute, Siberian Branch of the Russian Academy of Medical Sciences, Tomsk
Russian Federation
5, Kooperativny Street, Tomsk-634050
Russian Federation
5, Kooperativny Street, Tomsk-634050
L. А. Tashireva
Cancer Research Institute, Siberian Branch of the Russian Academy of Medical Sciences, Tomsk
Russian Federation
5, Kooperativny Street, Tomsk-634050
Russian Federation
5, Kooperativny Street, Tomsk-634050
О. V. Savenkova
Cancer Research Institute, Siberian Branch of the Russian Academy of Medical Sciences, Tomsk
Russian Federation
5, Kooperativny Street, Tomsk-634050
Russian Federation
5, Kooperativny Street, Tomsk-634050
Е. V. Kaigarodova
Cancer Research Institute, Siberian Branch of the Russian Academy of Medical Sciences, Tomsk
Russian Federation
5, Kooperativny Street, Tomsk-634050
Russian Federation
5, Kooperativny Street, Tomsk-634050
G. S. Zhamgaryan
Cancer Research Institute, Siberian Branch of the Russian Academy of Medical Sciences, Tomsk
Russian Federation
5, Kooperativny Street, Tomsk-634050
Russian Federation
5, Kooperativny Street, Tomsk-634050
References
1. Bulanov D.V., Mahson A.N. The immunohistochemical characteristics and prognostic criteria of Ewing’s sarcoma/PNET // Rossijskij onkologicheskij zhurnal. 2010. № 1. P. 17–19. [in Russian]
2. Vasil’ev N.V. Synovial sarcoma. Prognostic assessment // Sibirskij onkologicheskij zhurnal. 2010. № 1. P. 73–78. [in Russian]
3. Kekeeva T.V., Rjazanceva A.A., Zavalishina L.Je., Andreeva Ju.Ju., Babenko O.V., Zaletaev D.V., Frank G.A. Analysis of SYT/SSX1 and SYT/ SSX2 fusion genes in synovial sarcoma // Molekuljarnaja biologija. 2011. Vol. 45 (5). P. 840–844. [in Russian]
4. Kekeeva T.V., Rjazanceva A.A., Zavalishina L.Je., Andreeva Ju.Ju., Babenko O.V., Zaletaev D.V., Frank G.A. Integrated molecular diagnosis of synovial sarcoma // Molekuljarnaja medicina. 2010. № 3. P. 43–47. [in Russian]
5. Amary M., Berisha F., Bernardi F.C., Herbert A., James M., ReisFilho J.S., Fisher C., Nicholson A.G., Tirabosco R., Diss T.C., Flanagan A.M. Detection of SS18-SSX fusion transcripts in formalin-Bxed parafinembedded neoplasms: analysis of conventional RT-PCR, qRT-PCR and dual color FlSH as diagnostic tools for synovial sarcoma // Mod. Pathol. 2007. Vol. 20 (4). P. 482–496.
6. Fletcher C.D.M., Bridge J.A., Hogendoorn P.C.W., Mertens F. WHO Classification of Tumours of Soft Tissue and Bone. IARC: Lyon, 2013. 468 p.
7. Hakky T.S., Gonzalvo A.A., Lockhart J.L., Rodriguez A.R. Primary Ewing sarcoma of the kidney: a symptomatic presentation and review of the literature // Ther. Adv. Urol. 2013. Vol. 5 (3). P. 153–159. doi: 10.1177/1756287212471095.
8. Hosono Т., Hironaka M., Kobayashi A., Yamasawa H., Bando M., Ohno S., Sohara Y., Sugiyama Y. Primary pulmonary synovial sarcoma confirmed by molecular detection of SYT-SSX1 Fusion Gene Transcripts: a case report and review of the literature // Jpn. J. Clin. Oncol. 2005. Vol. 35 (5). P. 274–279.
9. Lopes H., Pereira C.A., Zucca L.E., Serrano S.V., Silva S.R., Camparoto M.L., Cárcano F.M. Primary monophasic synovial sarcoma of the kidney: a case report and review of literature // Clin. Med. Insights Oncol. 2013. Vol. 7 (7). P. 257–262. doi: 10.4137/CMO.S12243.
10. Olsen S.H., Thomas D.G., Lucas D.R. Cluster analysis of immunohistochemical profiles in synovial sarcoma, malignant peripheral nerve sheath tumor, and Ewing sarcoma // Mod. Pathol. 2006. Vol. 19 (5). P. 659–668.
11. White B.E., Kaplan A., Lopez-Terrada D.H., Ro J.Y., Benjamin R.S., Ayala A.G. Monophasic synovial sarcoma arising in the vulva // Arch. Pathol. Lab. Med. 2008. Vol. 132. P. 698–702. doi: 10.1043/1543-2165.
Review
For citations:
Perelmuter V.М., Vasilyev N.V., Tashireva L.А., Savenkova О.V., Kaigarodova Е.V., Zhamgaryan G.S. GENE EXPRESSION PROFILE AND MOLECULAR-GENETIC ANALYSIS OF SYNOVIAL SARCOMA AND EWING’ SARCOMA/PNET. Siberian journal of oncology. 2014;(2):19-23. (In Russ.)
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