EFFECT OF PULSE PERIODIC X-RAYS ON TUMOR AND BONE MARROW CELLS OF MICE
Abstract
The results of the effect of pulse-periodic X-ray exposure (PPXE) generated by Sinus-150 accelerator on tumor and normal cells of mice have been presented. Chromosomal aberrations (metaphase analysis) of bone marrow cells were studied. Using the cultured Lewis lung carcinoma cell lines, the level of apoptotic cells and cells with high concentration of active oxygen forms (AOF) was assessed by the flow cytofluorometry analysis (FACSCantoII, BD). In vivo X-ray radiation in the pulse-periodic mode lead to the moderate increase in chromosomal aberrations of bone marrow cells of mice. PPXE induced apoptotic death in Lewis lung carcinoma cells in vitro due to AOF production. Dose-effect relation was not observed at the irradiation doses 0.2–1.0 Gy. The data obtained show that further studies are necessary to justify the use of PPXE in tumor therapy.
About the Authors
М. А. Buldakov
Tomsk Cancer Research Institute
Tomsk State University, Tomsk
Russian Federation
5, Kooperativny Street, 634050-Tomsk
Russian Federation
5, Kooperativny Street, 634050-Tomsk
N. V. Litvyakov
Tomsk Cancer Research Institute
Tomsk State University, Tomsk
Russian Federation
5, Kooperativny Street, 634050-Tomsk
Russian Federation
5, Kooperativny Street, 634050-Tomsk
I. А. Klimov
Tomsk Cancer Research Institute
Russian Federation
Russian Federation
О. P. Kutenkov
Russian Federation
А. А. Melnikov
Tomsk Cancer Research Institute
Russian Federation
Russian Federation
М. А. Bolshakov
Institute of High-Current Electronics, Siberian Division, Russian Academy of Sciences, Tomsk
Tomsk State University, Tomsk
Russian Federation
5, Kooperativny Street, 634050-Tomsk
Russian Federation
5, Kooperativny Street, 634050-Tomsk
V. V. Rostov
Institute of High-Current Electronics, Siberian Division, Russian Academy of Sciences, Tomsk
Tomsk State University, Tomsk
Russian Federation
5, Kooperativny Street, 634050-Tomsk
Russian Federation
5, Kooperativny Street, 634050-Tomsk
N. V. Cherdyntseva
Tomsk Cancer Research Institute
Tomsk State University, Tomsk
Russian Federation
5, Kooperativny Street, 634050-Tomsk
Russian Federation
5, Kooperativny Street, 634050-Tomsk
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Review
For citations:
Buldakov М.А., Litvyakov N.V., Klimov I.А., Kutenkov О.P., Melnikov А.А., Bolshakov М.А., Rostov V.V., Cherdyntseva N.V. EFFECT OF PULSE PERIODIC X-RAYS ON TUMOR AND BONE MARROW CELLS OF MICE. Siberian journal of oncology. 2014;(5):34-38. (In Russ.)
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