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ROLE OF TRANSFORMING GROWTH FACTOR RECEPTOR B I TYPE (TGF-BRI) IN THE PROGRESSION OF THE LUMINAL BREAST CANCER SUBTYPE

https://doi.org/10.21294/1814-4861-2017-16-2-27-35

Abstract

Introduction. The crosstalk between the estrogen and growth factor receptors signaling may play an important role in the resistance to endocrine therapy. The aim of the study was to examine the relationship between the protein and receptor gene expression of transforming growth factor β type I (TGF-βRI) and its polymorphism with progression of luminal breast cancer patients treated by adjuvant tamoxifen.

Material and methods.  The study included 105 patients with luminal breast cancer (T1-3N0–3M0), who had received adjuvant tamoxifen  at a dose of 20 mg/day for at least 5 years. Patients who developed distant metastasis or recurrence after tamoxifen therapy were defined as tamoxifen resistance (TR) group, while distant metastasis-free patients were analyzed as tamoxifen sensitive (TS) group. TGF-βRI expression level was evaluated using immunohistochemistry. TGF-BRI gene expression and genotypes for rs334354 SNP were detected by a Real-time PCR.

Results. We found high TGF-βRI gene expression in patients with luminal A subtype compared with luminal B breast cancer (p=0.050). The Int7G24AA and Int7G24A mutant carriers were more prevalent in luminal A breast cancer patients (p=0.019 and p=0.007, respectively). TGF-βRI protein expression level was significantly higher in the tamoxifen sensitive group compared to tamoxifen resistance breast cancer patients regardless of molecular subtypes (p=0.043). There was a trend for a tamoxifen sensitivity among luminal B breast cancer patients with a high TGF-βRI protein expression (p=0.090).

Conclusion. TGF-βRI protein expression level can be a potential molecular marker of tamoxifen resistance in luminal breast cancer patients.

About the Authors

N. N. Babyshkina
Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences; National Research Tomsk State University
Russian Federation

Babyshkina Natalia N. - MD, PhD, Senior Research Scientist, Laboratory of Molecular Oncology and Immunology, CRI, TNRMC RAS.

5, Kooperativny Street, 634009, Tomsk; 36, Lenina, Street, 634050, Tomsk. -mail: nbabyshkina@mail. ru. SPIN-code: 2738-9275



S. V. Vtorushin
Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences; Siberian State Medical University
Russian Federation

Vtorushin Sergey V. - MD, Professor, Department of Anatomical Pathology, SSMU.

5, Kooperativny Street, 634009, Tomsk; 2, Moskovsky Tract Street, 634050, Tomsk. E-mail: wtorushin@rambler.ru. SPIN-code: 2442-4720



T. A. Dronova
National Research Tomsk State University
Russian Federation

Dronova Tatiana A. – postgraduate.

36, Lenina, Street, 634050, Tomsk. E-mail: tanyadronova@mail.ru. SPINcode: 3516-2517



N. V. Krakhmal
Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences; Siberian State Medical University
Russian Federation

Krakhmal Nadezhda V. - MD, PhD, Assistant, Department of Anatomical Pathology, SSMU.

5, Kooperativny Street, 634009, Tomsk; 2, Moskovsky Tract Street, 634050, Tomsk. E-mail: krakhmal@mail.ru. SPIN-code: 1543-6546



M. V. Zavyalova
Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences; National Research Tomsk State University; Siberian State Medical University
Russian Federation

Zavyalova Marina V. - MD, Professor, Senior Researcher, Department of Pathological Anatomy and Cytology, Tomsk Cancer Research Institute, Head of Pathological Anatomy Department, SSMU.

5, Kooperativny Street, 634009, Tomsk; 36, Lenina, Street, 634050, Tomsk; 2, Moskovsky Tract Street, 634050, Tomsk. E-mail: zavyalovamv@mail. ru. SPIN code: 1229-0323



M. M. Tsyganov
Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences; National Research Tomsk State University
Russian Federation

Tsyganov Matvey M. - Junior Research, Laboratory of Viral Oncology, Cancer Research Institute, TNRMC RAS.

5, Kooperativny Street, 634009, Tomsk; 36, Lenina, Street, 634050, Tomsk. E-mail: TsyganovMM@yandex.ru. SPIN-code: 1253-0240



S. V. Patalyak
Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences
Russian Federation

Patalyak Stanislav V. - MD, PhD, Researcher, General Oncology Department, Cancer Research Institute, TNRMC RAS.

5, Kooperativny Street, 634009, Tomsk, E-mail: Patalyak@gmail.com. SPIN-code: 8497-1750



E. M. Slonimskaya
Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences; Siberian State Medical University
Russian Federation

Slonimskaya Elena M. - MD, Professor, Head of General Oncology Department, Cancer Research Institute, TNRMC RAS.

5, Kooperativny Street, 634009, Tomsk; 2, Moskovsky Tract Street, 634050, Tomsk. E-mail: slonimskaya@rambler.ru. SPIN-code: 7763-6417



N. V. Cherdyntseva
Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences; National Research Tomsk State University
Russian Federation

Cherdyntseva Nadezhda V. - Associate Member of Russian Academy of Sciences, PhD, Professor, Deputy Director for Basic Science, Head of Laboratory of Molecular Oncology and Immunology, Cancer Research Institute, TNRMC RAS.

5, Kooperativny Street, 634009, Tomsk; 36, Lenina, Street, 634050, Tomsk. E-mail: nvch@oncology.tomsk.ru. SPIN-code: 5344-0990 



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Review

For citations:


Babyshkina N.N., Vtorushin S.V., Dronova T.A., Krakhmal N.V., Zavyalova M.V., Tsyganov M.M., Patalyak S.V., Slonimskaya E.M., Cherdyntseva N.V. ROLE OF TRANSFORMING GROWTH FACTOR RECEPTOR B I TYPE (TGF-BRI) IN THE PROGRESSION OF THE LUMINAL BREAST CANCER SUBTYPE. Siberian journal of oncology. 2017;16(2):27-35. (In Russ.) https://doi.org/10.21294/1814-4861-2017-16-2-27-35

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ISSN 1814-4861 (Print)
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